Molecular and Epidemiological Studies on Maize Army Worm Densovirus by Dr. Rabab A.A. El-Mergawy
Molecular and Epidemiological Studies on Maize Army Worm Densovirus by Dr. Rabab A.A. El-Mergawy PDF, ePub eBook D0wnl0ad
The present work was carried out to Clarify the noctuid fauna of lucern in El-Bahareya oasis, determine the population fluctuation of surveyed noctuid species, determine the efficiency of MlDNV against the 2nd, 3rd and 4th larval instars of Spodoptera littoralis, investigate the natural presence of densovirus in different Egyptian geographical places and study the natural moleculr diversity of some isolates. Results indicated remarkable infestations with Seven different noctuid species, A. segetum, A. ipsilon, A. spinifera, H. armigera, A. gamma, S. exigua. and S. littoralis. The observed fluctuation of the population showed that S. littoralis larvae reached its highest average of the catch in June but one infestation peak could be detected around mid May, S. exigua larvae reached its highest average of the catch in April and May, and two infestation peaks could be detected around May and June. The larvae of H. armigera were found appeared during the summer and the beginning of the autumn, where it reached its highest average of the catch in October and two infestation peaks around July and September could be detected. The larvae of the cutworms (Agrotis spp.) were found appeared during the end of autumne and all the winter. The highest average of the catch of the larvae of A. ipsilon was during January while it was in May for A. segetum. Bioassay of MlDNV were conducted against the 2nd, 3rd and 4th larval instars of S. littoralis. The values of LC50 were estimated respectively by 0.054, 0.095 and 0.191OD/ml. Screenings attempts using the total nucleic probe test and PCR amplification revealed the presence of Densovirus in the 7 surveyed noctuide species in lucern and cotton fields in different Egyptian geographical localities. Characterization of the virions and the restriction profile of the isolated viruses showed no differences between these isolates and MlDNV previousely isolated in Egypt, which suggested that those viruses are different isolates of MlDNV. Result of the sequencing of the capsid gene encoding the viral polypeptides (VP) of 7 different isolates from S. exigua and S. littoralis larvae collecting from El-Bahareya Oasis was carried out comparing with the MlDNV VP, revealed 3 mutations, which suggested that those viruses are different strains of MlDNV. A new Densonucleosis virus was isolated in El-Bahareya Oasis from H. armigera, it was named by HaDNV. HaDNV appeared as icosahedral non-enveloped particles of 25nm in diameter. Immunodiffusion test, revealed a serological ralationship between HaDNV and MlDNV. The virions of HaDNV contains the four structural proteins related to MlDNV, 91, 63, 53 and 47KDa respectively, and two extra structural proteins with molecular weight of 40 and 41KDa. The average size of HaDNV DNA molecule was estimated to be 5.95 kb. Restriction profiles of the HaDNV DNA showed essential differences between HaDNV and MlDNV. Partial sequencing for the clones of HaDNV DNA and MlDNV DNA, revealed 89 % identity between the NS region of HaDNV DNA and MlDNV DNA and 97% identity between the ITR region of HaDNV DNA. It was suggested that HaDNV is a new member of the Densovirus genus (parvoviridae family). The results showed the high efficiency, polyspecifity and the natural presence of MlDNV in the natural agricultural Egyptian environment, which confirm on the importance of MlDNV as a biocontrol agent in case of it was confirmed as safe agent for non-target organisms. The results also confirmed on the biodiversity of densoviruses.From reader reviews:
Jose Higham:
Now a day individuals who Living in the era just where everything reachable by connect with the internet and the resources inside it can be true or not require people to be aware of each information they get. How individuals to be smart in having any information nowadays? Of course the solution is reading a book. Reading through a book can help men and women out of this uncertainty Information specifically this Molecular and Epidemiological Studies on Maize Army Worm Densovirus book since this book offers you rich details and knowledge. Of course the knowledge in this book hundred per cent guarantees there is no doubt in it everbody knows.
Kendrick Hardee:
The book Molecular and Epidemiological Studies on Maize Army Worm Densovirus will bring that you the new experience of reading any book. The author style to clarify the idea is very unique. In case you try to find new book to read, this book very acceptable to you. The book Molecular and Epidemiological Studies on Maize Army Worm Densovirus is much recommended to you to see. You can also get the e-book from official web site, so you can quicker to read the book.
James Bouchard:
You may get this Molecular and Epidemiological Studies on Maize Army Worm Densovirus by visit the bookstore or Mall. Just simply viewing or reviewing it could possibly to be your solve issue if you get difficulties on your knowledge. Kinds of this e-book are various. Not only by simply written or printed but additionally can you enjoy this book by means of e-book. In the modern era just like now, you just looking by your local mobile phone and searching what their problem. Right now, choose your own ways to get more information about your e-book. It is most important to arrange you to ultimately make your knowledge are still revise. Let's try to choose proper ways for you.
Read Molecular and Epidemiological Studies on Maize Army Worm Densovirus by Dr. Rabab A.A. El-Mergawy for online ebook
Molecular and Epidemiological Studies on Maize Army Worm Densovirus by Dr. Rabab A.A. El-Mergawy Free PDF d0wnl0ad, audio books, books to read, good books to read, cheap books, good books, online books, books online, book reviews epub, read books online, books to read online, online library, greatbooks to read, PDF best books to read, top books to read Molecular and Epidemiological Studies on Maize Army Worm Densovirus by Dr. Rabab A.A. El-Mergawy books to read online.
No comments:
Post a Comment